The potential of the three-spined stickleback (Gasterosteus aculeatus L.) as a combined biomarker for oestrogens and androgens in European waters.

Detection and assessment of androgenic potency of endocrine-disrupting chemicals using three-spined stickleback, Gasterosteus aculeatus.

The male three-spined stickleback (Gasterosteus aculeatus) produces a glue protein named "spiggin" that is used as a cementing substance for building its nest. The synthesis of spiggin is under the control of androgenic stimulation. Therefore, spiggin is an effective biomarker of any androgenic activity displayed by environmental chemicals, similarly to the use of vitellogenin as an estrogenic ...

A new ELISA for the three-spined stickleback (Gasterosteus aculeatus L.) spiggin, using antibodies against synthetic peptide.

The aim of this study was to develop an enzyme-linked immunosorbent (ELISA) assay to quantify spiggin in the three-spined stickleback. Spiggin is a glue protein produced in the kidney of male three-spined stickleback under the control of androgens during the breeding period. Disturbances of spiggin production in male fish and abnormal induction of spiggin in female fish are considered as valuab...

Geography of the circadian gene clock and photoperiodic response in western North American populations of the three-spined stickleback Gasterosteus aculeatus.

Controlled laboratory experiments were used to show that Oregon and Alaskan three-spined stickleback Gasterosteus aculeatus, collected from locations differing by 18° of latitude, exhibited no significant variation in length of the polyglutamine domain of the clock protein or in photoperiodic response within or between latitudes despite the fact that male and female G. aculeatus are photoperiod...

Induction of Cytochrome P450 1A (CYP1A) in larvae of the three-spined stickleback (Gasterosteus aculeatus L.)

Gonadal effects of melatonin in the three-spined stickleback, Gasterosteus aculeatus L., during different seasons and photoperiods.

Males and females of the three-spined stickleback, Gasterosteus aculeatus L., were given melatonin injections (0.8 or 4 micrograms/day) at different seasons and under different photoperiods (8L: 16D and 16L: 8D). Long photoperiods stimulated reproduction in November and January, whereas maturation in both sexes in March/April and ovarian regression in July were only negligibly affected by light...